Development of GABA-immunoreactive cells in the spinal cord of the sea lamprey, P. marinus.

Abstract

The lamprey spinal cord increases in length and size during all its life cycle; thus, it is expected that new cells will be generated. This expectation suggests that the locomotor circuits must be continuously remodeled. Key elements in the cellular network controlling locomotor behavior are inhibitory cells. Here, we studied the gamma-aminobutyric acid-immunoreactive (GABA-ir) cells in the lamprey spinal cord during postembryonic development. Three major populations of GABA-ir cells were identified according to their distribution: those located in the gray matter, those contacting the cerebrospinal liquid (LC cells), and those located in the white matter. The results show (1). the number of GABA-ir cells per segment increase from prolarvae (<10 mm) to adulthood; (2). the lower number of GABA-ir cells in 100 microm of spinal cord is 66 +/- 7, found in premetamorphic larvae, and the highest is 107 +/- 6, found in postmetamorphic animals; (3). the gray matter and LC GABA-ir cells show different variations in number depending on the developmental period. Thus, in the 10-mm larvae, the gray matter GABA-ir cells are more abundant than LC cells, whereas in the young postmetamorphic specimens, the contrary occurs. Most of the GABA-ir cells located in the white matter were classified as edge cells. They increase in number from the beginning of the prolarval period, where there are not white matter-positive cells, to the middle larval period, where there are 9 +/- 4 GABA-ir edge cells per segment. This value was unaltered in later periods, where GABA-ir edge cells represent 20-30% of the total number of edge cells per segment. The increase in number of GABA-ir cells in these populations during a specific point of the lamprey life cycle may indicate different inhibitory requirements of the locomotor circuit at different developmental periods.