Abstract
BackgroundDevelopment of sensitive and specific imaging approaches for the detection of ovarian cancer holds great promise for improving the therapeutic efficacy and the lifespan of the patients.ResultsIn this study, manganese-nitrogen doped carbon nanosheets (Mn-N-CNSs) coupled with Anti-HE4 monoclonal antibody (Mn-N-CNSs@Anti-HE4) were synthesized for the specific and targeted fluorescence/MR dual-modal imaging of ovarian carcinoma. The prepared Mn-N-CNSs revealed excellent aqueous dispersity, good colloidal stability, great optical properties and high longtudinal relaxivity rate (r1 = 10.30 mM−1 s−1). Encouraged by the tunable photoluminiscence of the nanoprobe and Anti-HE4 targeting ligand, the ovarian carcinoma cells were specifically labeled by the Mn-N-CNSs@Anti-HE4 nanoprobe with multi-color fluorescences. Benefiting from the high r1 relaxivity, the nanoprobe exhibited targeted and enhanced MR contrast effect in the ovarian carcinoma cells and tumor bearing mice model. Besides, the high biocompatibility and easy excretion from the body of the nanoprobe were further confirmed in vivo.ConclusionThe prepared Mn-N-CNSs@Anti-HE4 with excellent biocompatibility, high-performance and superior tumor-targeting ability provides a novel fluorescence/MR dual-modal nanoprobe for specific labeling and detection of ovarian carcinoma cells in vitro and in vivo.
Highlights
Development of sensitive and specific imaging approaches for the detection of ovarian cancer holds great promise for improving the therapeutic efficacy and the lifespan of the patients
Control experiments showed that the reaction time, temperature, and Mn2+ dosage have a great influence on the fluorescence and magnetic resonance imaging (MRI) properties of MnN-CNSs ( Additional file 1: Fig. S1)
In vivo targeted MR imaging Encouraged by the excellent performance of in vitro MR imaging, we investigated the ability of molecular MRI with the Mn-N-CNSs@Anti-human epididymis protein 4 (HE4) nanoprobe in imaging the ovarian carcinoma in HO8910-tumor-bearing mice
Summary
Development of sensitive and specific imaging approaches for the detection of ovarian cancer holds great promise for improving the therapeutic efficacy and the lifespan of the patients. Developing a imaging probe to realize early and accurate diagnosis of the ovarian carcinoma is urgently needed for improving the therapeutic efficacy and the lifespan of the patients [5]. Multimodal imaging based on molecular nanoprobe has gained increasing interests for the specific and accurate diagnosis of the tumor [6,7,8,9]. It could integrate the merits of different imaging techniques to improve the effectiveness and sensitivity of the diagnosis. The combination of MRI and FL imaging renders a promising dual-modal imaging technique with complementary characteristics to improve the accuracy of tumor diagnosis [11,12,13]
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