Abstract
Rock bream (Oplegnathus fasciatus) is one of the most economically valuable fish in Korea. In recent years, artificial breeding techniques with molecular and microsatellite markers have been developed to enhance rock bream resources. Microsatellite loci to define genetic diversity were screened in rock bream (n = 30) from Jeju areas of Korea and fifteen polymorphic microsatellite loci were newly identified and analyzed. The number of alleles per locus ranged from 9 to 34 while observed and expected heterozygosity ranged from 0.600 to 1.000 and from 0.772 to 0.977, respectively. These markers will serve as a foundation for future population genetic studies and the selective breeding technology of rock bream farming.
Highlights
Rock bream (Oplegnathus fasciatus) is primarily an inhabitant of estuaries throughout Korea, Japan, Taiwan, and Hawaii [1] [2]
We reported on an additional 15 microsatellite markers for the rock bream
All loci from our study show highly informative values, indicating that all loci from our research could be useful markers for population genetics and quantitative trait locus (QTL) study of rock bream
Summary
Rock bream (Oplegnathus fasciatus) is primarily an inhabitant of estuaries throughout Korea, Japan, Taiwan, and Hawaii [1] [2]. Many rock bream farms in Korea have suffered substantial financial loss due to the occurrence of red seabream iridovirus disease (RSIVD) during summer months [15] [16]. To reduce this economic loss, we carry out experiments to isolate fish with iridovirus resistance. For this purpose, the high resolution of genetic map is necessary to facilitate searching for disease resistance traits and identifying candidate genes. We reported on an additional 15 microsatellite markers for the rock bream
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