Abstract
To propose an experimental burn model in NIH-3T3 cell line. Induction of thermal injury in cultures of mouse fibroblast - NIH-3T3- cell line and determination of cell viability by MTT and immunofluorescence. The heating of the Petri dish increased proportionally to the temperature of the base and the time of exposure to microwave. In this in vitro burn model, using the cell line NIH-3T3 was observed drastic cellular injury with significant changes in cell viability and activity. It showed drastically modified cell morphology with altered membrane, cytoskeleton and nucleus, and low cellularity compared to the control group. The burn model in vitro using the cell line NIH-3T3 was reproductive and efficient. This burn model was possible to determine significant changes in cell activity and decreased viability, with drastic change in morphology, cell lysis and death.
Highlights
Burns are responsible for many pathophysiological changes[1,2,3], represents a severe form of trauma[3,4]
There are many advances in knowledge of burn care, treatment is still suboptimal, because there is still lack of studies based on evidence[5]
The objective of this study is to propose an experimental burn model of cultured fibroblasts
Summary
Burns are responsible for many pathophysiological changes[1,2,3], represents a severe form of trauma[3,4]. There are many advances in knowledge of burn care, treatment is still suboptimal, because there is still lack of studies based on evidence[5]. In experimental in vivo models it is difficult to characterize molecular changes by thermal burns at the cellular level in isolation, since there are many variables involved. Due to the systemic response by thermal injury, especially inflammatory and coagulation cascades, it is difficult to assess only the cellular effect produced by direct stimulation[8]. The objective of this study is to propose an experimental burn model of cultured fibroblasts
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