Abstract
Summary Sandwich elisa were developed to quantitatively determine conglutinin (cg), mannan-binding protein (mbp), and serum amyloid-P component (sap) in the sera of cattle. The elisa system was found to have high repeatability for quantitation of these serum proteins at concentration as low as 5 ng/ml. From results obtained for 10 healthy cows aged 2 to 7 years, mean ± sd serum concentrations were 56.5 ± 14.4 μg of cg/ml, 2.37 ± 0.87 μg of mbp/ml, and 11.14 ± 3.92 μg of sap/ml, respectively. Values in 6 healthy heifer calves aged 6 months were 3.45 ± 1.22 μg/ml for cg, 1.71 ± 0.96 μg/ml for mbp, and 5.45 ± 2.75 μg/ml for sap, respectively. Concentrations in 9 healthy bullocks aged 6 months were 1.83 ± 0.66 μg/ml for cg, 1.04 ± 0.63 μg/ml for mbp, and 4.9 ± 1.13 μg/ml for sap, respectively.
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