Abstract
Non-small cell lung cancer (NSCLC) is the most common type of lung cancer accounting for 85% of all newly diagnosed cases. Its prognosis remains poor as most patients are diagnosed at an advanced stage. In this study, we report the development of an electrochemical immunosensor for quantitative detection of Yamaguchi sarcoma viral oncogene homolog 1 (v-YES1) protein, comprised of a glassy carbon electrode modified with gold nanoparticles (AuNP), thiolated protein G (TPG), YES1 antibody (AB1) and glutaraldehyde (GA), which was used as a cross linker. Cyclic voltammetry (CV) and Differential pulse voltammetry (DPV) were used to measure the response and characterization of the fabricated immunosensor. The fabricated immunosensor, glassy carbon electrode (GCE)/AuNP/TPG/GA/Ab1) was optimized for pH, response time, antibody concentration and temperature. Under optimum conditions, the immunosensor displayed high sensitivity, recording a limit of detection (LOD) of 0.0014 ng/mL and was noted to have negligible cross reactivity. The proposed immunosensor proved to be stable for up to 2 weeks, which means that it can be used as an alternative diagnostic tool for the rapid, sensitive and specific detection of YES1 antigen in clinical samples for clinical monitoring of cancer progression.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
