Development of Eighteen Microsatellite Markers in Anemone amurensis (Ranunculaceae) and Cross-Amplification in Congeneric Species

Mingzhou Sun,Lin Li,Xiao Yin,Fengxue Shi,Linfeng Li,Hongxing Xiao,Mingrui Li

doi:10.3390/ijms13044889

Mingzhou Sun, Lin Li + Show 5 more

Open Access

https://doi.org/10.3390/ijms13044889

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Abstract

Polyploidy plays an important role in the evolution of plant genomes. To enable the investigation of the polyploidy events within the genus Anemone, we developed eighteen microsatellite markers from the hexaploid species A. amurensis (Ranunculaceae), and tested their transferability in five closely related species. The number of total alleles (NA) for each resulting locus varied from one to eight. The polymorphism information content (PIC) and Nei’s genetic diversity (NGD) for these microsatellites ranged from 0.00 to 0.71 and 0.00 to 0.91, respectively. For each population, the NA was one to seven, and the values of PIC and NGD varied from 0.00 to 0.84 and 0.00 to 0.95, respectively. In addition, most of these microsatellites can be amplified successfully in the congeneric species. These microsatellite primers provide us an opportunity to study the polyploid evolution in the genus Anemone.

Highlights

Polyploidy has been recognized as a pervasive force in plant evolution, and more than half of flowering plants have a polyploid ancestry [1,2]
In order to evaluate the polyploidy events within the genus Anemone, we investigated an annual herbaceous plant of northeastern China, namely Anemone amurensis Kom. (Ranunculaceae)
The locus HS199 showed a significant departure (p < 0.01) from Hardy-Weinberg equilibrium (HWE) under the diploid model and no significantly (p < 0.01) linkage disequilibrium was detected for any pair of loci

Summary

Introduction

Polyploidy has been recognized as a pervasive force in plant evolution, and more than half of flowering plants have a polyploid ancestry [1,2] Those polyploidy events contributed greatly to the diversification and evolution of angiosperms [3,4]. The species A. amurensis has a hexaploid genome, its closely related species were found to have diploid and tetraploid genomes. These attributes indicate that the genus Anemone is an ideal system to study polyploidy. We developed eighteen microsatellite markers from A. amurensis and tested their transferability in other congeneric species. These microsatellites provide us with an opportunity to study polyploidy events within the genus Anemone

Results and Discussion

Isolation of Microsatellite Markers

Detection of Polymorphism and Data Analysis

Conclusions