Development of dwarfish and yield-effective GM maize through passivation of bioactive gibberellin.

Abstract

During the Green Revolution in the 1960s, breeding dwarf cultivars turned out to be a landmark, leading to a significant increase in the global production of wheat and rice. The most direct and effective strategy for breeding dwarf crops, among others, would be to control endogenous gibberellin (GA) levels of the crops. GA 2-oxidases are a group of 2-oxoglutarate-dependent dioxygenases that catalyze the deactivation of bioactive GAs. The ArabidopsisAtGA2ox1 gene was transformed into maize with the aim of obtaining a height-reduced GM maize. The characterization of the GM maize revealed that the highest plant height reduction was accomplished by a 74% decline in GA1 level, and by approximately twofold increases in both chlorophyll content and root/shoot ratio over the wild-type (WT). Interestingly, the stem cells of the GM maize were condensed, and the typical vascular bundle structure was found to be deformed. Based on a 2-season field trial, the GM maize exhibited a higher harvest index (9-17%) and grain yield (10-14%) than the WT. The current results suggest that a modulation of the endogenous GA level would be a sensible approach for improving the crop architecture and grain yield in maize.