Abstract
In recent years, Siniperca chuatsi rhabdovirus (SCRV) caused serious threats and huge economic losses in Siniperca chuatsi aquaculture industry. Vaccination is the most efficacious and cost-effective strategy to control this viral disease. However, SCRV-QY vaccine quality control is vital for successful prevention. Herein, we generated a pair of high affinity antibodies against SCRV-QY virus and established a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for detecting the SCRV-QY antigen amounts. In this assay, monoclonal antibody 4H8 was selected as capture antibody and 4E12 labeled HRP for detector antibody. A standard curve was generated using the SCRV concentration versus OD value with the linear range of concentration of 78.125~5000 ng/ml. The antigen content of 3 batches SCRV-QY inactivated vaccines were quantitatively detected by using the DAS-ELISA. The results showed that antigen contents of SCRV-QY inactivated vaccines were positively correlated with the viral titers. In conclusion, this DAS-ELISA was an accurate, quick and efficacious method for detecting antigen concentration of inactivated SCRV-QY vaccines.
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