Abstract
A DNA hybridization dot-blot assay using a radioactive and a non-radioactive probe has been developed for the detection of Aujeszky's disease virus (ADV). The Bam H I 7 fragment of ADV genomic DNA was labeled by nick translation using 32P-dCTP and the 196bp polymerase chain reaction (PCR) gG glycoprotein gene amplified fragment was also labeled by nick translation but using biotin d-7-dATP. This technique provides a fast and effective means of detecting acute cases of ADV infection but it was unable to detect ADV nucleic acid sequences in trigeminal nerve ganglia of latent infected pigs and mice.
Highlights
Aujeszky's disease virus (ADV) is the causative agent of disease in pigs, weaners and fatteners in which the mortality rate may vary widely, depending upon the age of the swine affected (Gustafson, 1986)
No signs of AD were seen in the infected pigs exposed to 2 ́105 TCID50 of the ASB Piau strain of ADV in each nostril
Amplification with primers LVC A1 and A2 yielded a product of approximately 260bp. This product was clearly visualized as a sharp band on agarose gels (Fig. 2A, 2B and 2C) on all trigeminal nerve ganglia collected in infected pigs 350 days after exposure, in group A mice three weeks after exposure, in group A mice three weeks after exposure to virulent virus and in group B mice at the time of death
Summary
Aujeszky's disease virus (ADV) is the causative agent of disease in pigs, weaners and fatteners in which the mortality rate may vary widely, depending upon the age of the swine affected (Gustafson, 1986). Pigs are considered to be the host of ADV a variety of animals may be infected and in most non swine animals the disease is usually fatal (Baskerville et al, 1973). A latent infection is established following the acute phase of the disease (Carneiro & Cardim, 1947; Kojnok, 1965). To diagnose acute ADV infection the palatine tonsil and trigeminal nerve ganglia are tested for the presence of virus by either fluorescent antibody technique, cell culture, blot hybridization or PCR. Determining the occurrence of latent ADV infections requires a sensitive assay system (Maes et al, 1988; Rziha et al, 1988; White et al, 1996)
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