Abstract
A novel amperometric biosensor prototype was fabricated using screen printing technique. The disposable single-use strips were made from conductive carbon ink and modified with fructosyl amino acid oxidase. The electrodes and conducting paths were made solely with carbon ink and characterized by conductivity and cyclic voltammetry. The biosensor showed high current output, large linearity, and effectiveness for fructosyl valine as well as human blood samples. Amperometric studies were carried out using both fructosyl valine and human blood samples. With 5 uL sample volume, the biosensor showed strong amperometric response with good linearity for a wide range (0 to 8 mM). Diabetic and healthy blood samples showed sufficient difference in their amperometric responses that correlate well with their different hemoglobin A1c levels. These results demonstrate the feasibility of using this type of inexpensive single-use biosensor strips as the basis for determining hemoglobin A1c levels for diabetic patients.
Highlights
Hemoglobin (Hb) is the oxygen-transport protein and hemoglobin A1 (HbA1) is the most abundant hemoglobin component in human erythrocytes
The disposable single-use strips were made from conductive carbon ink and modified with fructosyl amino acid oxidase
Diabetic and healthy blood samples showed sufficient difference in their amperometric responses that correlate well with their different hemoglobin A1c levels. These results demonstrate the feasibility of using this type of inexpensive single-use biosensor strips as the basis for determining hemoglobin A1c levels for diabetic patients
Summary
Hemoglobin (Hb) is the oxygen-transport protein and hemoglobin A1 (HbA1) is the most abundant hemoglobin component in human erythrocytes. Trial (DCCT) has established the relationship between HbA1c level and the risks for development and progression of chronic complications of diabetes. In this paper we report the development of new single-use strips for A1c determination. Chawla and Pundir reported using FAO modified gold electrode for quantifying HbA1c [17]. It wasn’t single use screen printed electrode (SPE) such as glucose strips. Fan et al reported FAO modified iridium electrode for quantifying HbA1c that was single-use SPE, there was no blood sample tested on the iridium SPE [18]. The SPEs have been tested on both fructosyl-valine and blood samples from healthy and diabetic subjects
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