Abstract
BackgroundA wild-abortive-type (WA) cytoplasmic male sterility (CMS) has been almost exclusively used for breeding three-line hybrid rice. Many indica cultivars are known to carry restorer genes for WA-CMS lines and cannot be used as maintainer lines. Especially elite indica cultivars IR24 and IR64 are known to be restorer lines for WA-CMS lines, and are used as male parents for hybrid seed production. If we develop CMS IR24 and CMS IR64, the combination of F1 pairs in hybrid rice breeding programs will be greatly broadened.FindingsFor production of CMS lines and restorer lines of IR24 and IR64, we employed Chinese wild rice (CW)-type CMS/Restorer of fertility 17 (Rf17) system, in which fertility is restored by a single nuclear gene, Rf17. Successive backcrossing and marker-assisted selection of Rf17 succeeded to produce completely male sterile CMS lines and fully restored restorer lines of IR24 and IR64. CW-cytoplasm did not affect agronomic characteristics.ConclusionsSince IR64 is one of the most popular mega-varieties and used for breeding of many modern varieties, the CW-CMS line of IR64 will be useful for hybrid rice breeding.Electronic supplementary materialThe online version of this article (doi:10.1186/s12284-016-0097-2) contains supplementary material, which is available to authorized users.
Highlights
A wild-abortive-type (WA) cytoplasmic male sterility (CMS) has been almost exclusively used for breeding three-line hybrid rice
Most commercial hybrid rice has been developed based on a three-line system, namely A (CMS line), B and R, involving the almost exclusive use of wild-abortive-type CMS (WA-CMS) lines, as it accounted for about 90 % of the rice hybrids produced in China and 100 % of the hybrids developed outside China (Sattari et al 2007)
Two-line hybrids based on photoperiod- or thermo-sensitive genic male sterile lines are gradually increased in China up to onethird of the total hybrid rice growing area, WA-CMS still predominated (Huang et al 2014)
Summary
A wild-abortive-type (WA) cytoplasmic male sterility (CMS) has been almost exclusively used for breeding three-line hybrid rice. A major technical handicap in the development of hybrid rice using WA-CMS is a limited source of maintainer lines, as many indica elite cultivars are known to carry restorer genes for WA-CMS lines and cannot be used as IR24 and IR64 are restorer lines for WA-CMS lines, and are used as male parents for hybrid seed production (Jing et al 2001; Cai et al 2013).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
