Abstract
ABSTRACTThe colloidal gold quick test card which we developed was used for rapid detection of Alicyclobacillus acidoterrestris (A. acidoterrestris) in apple juice concentrate. Two antibodies against A. acidoterrestris, obtained from Sprague Dawley (SD) rats and Japan White Rabbits, were adopted to construct this quick test card. The results showed that the colloidal gold quick test card had better specificity, and was more rapid and convenient which deserved notice especially (the test could be completed within 5–10 min) and concorded with ELISA method (we previously report) and K medium methods (traditional classical method). This new developed colloidal gold quick test card (we named it TAB quick test card) could be more convenient for apple juice safety testing.
Highlights
Alicyclobacillus acidoterrestris is a thermophilic, acidophilic, and spore-forming bacterium (TAB) in many fruit juices (Cerny, Hennlich, & Poralla, 1984), such as apple, pear, orange, citrus, and so on
The colloidal gold quick test card which we developed was used for rapid detection of Alicyclobacillus acidoterrestris (A. acidoterrestris) in apple juice concentrate
The results showed that the colloidal gold quick test card had better specificity, and was more rapid and convenient which deserved notice especially and concorded with ELISA method and K medium methods
Summary
Alicyclobacillus acidoterrestris is a thermophilic, acidophilic, and spore-forming bacterium (TAB) in many fruit juices (Cerny, Hennlich, & Poralla, 1984), such as apple, pear, orange, citrus, and so on. The reverse-transcription polymerase chain reaction (RT-PCR) detection by Yamazaki, Teduka, Inoue, and Shinano (1996), a realtime PCR rapid detection method by Luo, Yousef, and Wang (2004), a quantitatively competitive PCR (QC-PCR) method by Li, Feng, and Qiu (2006), and a Fourier transform infrared (FT-IR) spectroscopy detection by Al-Qadiri, Lin, Cavinato, and Rasco (2006) Those methods are difficult to use in factory because it requires skilled personnel and relatively expensive equipments (Li, Huang, Xia, & Liu, 2014). A new colloidal gold quick card (TAB quick test card) was developed with the aid of colloidal gold immunochromatographic assay This test card showed more rapidness and convenience for rapid detection of A. acidoterrestris in apple juice
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