Development of clinically relevant orthotopic xenograft mouse model of metastatic lung cancer and glioblastoma through surgical tumor tissues injection with trocar

Xi Feng Fei,Zi Cheng Wu,Ru Jun Li,Qiang Huang,Zhi Min Wang,Ai Dong Wang,Quan Bin Zhang,Qing Lan,Shi Ming Zhang,Jun Dong,Yi Diao

doi:10.1186/1756-9966-29-84

Abstract

ObjectiveOrthotopic models are important in cancer research. Here we developed orthotopic xenograft mouse model of metastatic lung cancer and glioblastoma with a specially designed system.MethodsTiny fragments of surgical tumors were implanted into the mice brain with a trocar system. Immunohistochemistry was performed to detect brain tumor stem cells among glioblastoma tissues, including both the original and resulting ones with monoclonal antibody against CD133.ResultsBesides the constant high take rates in both models; brain transplants perfectly resembled their original tumors in biological behaviors. The brain tumor stem cells, positively stained with CD133 were found, though not frequently, in both original and resulting glioblastoma tissues.ConclusionsOrthotopic model established with a trocar system is effective and injection of tumor tissues containing stem cells promise the forming of new tumor mass when grafted.

Highlights

Animal models have been extremely critical in the understanding of cancer and in the pre-clinical testing of new antitumor drugs since 1960s when it was first developed by implanting human colon carcinoma to nude mice [1]
We developed orthotopic xenograft mouse model by injecting tiny tumor tissue, but not cell suspensions, into the brain of mouse with a special trocar system
We examined the distribution of CD133+ cells in both the original and implanted tumors of glioblastoma multiforme

Summary

Introduction

Animal models have been extremely critical in the understanding of cancer and in the pre-clinical testing of new antitumor drugs since 1960s when it was first developed by implanting human colon carcinoma to nude mice [1]. The results indicated that though the extent to which murine models recapitulate. As far as human brain tumors are concerned, the orthotopic models currently available are established either by stereotaxic injection of cell suspensions [5,6,7,8] or implantation in solid piece through complicated craniotomy [9,10]. Taking into consideration both the advantages and disadvantages of the current methods, there is still much room for improvement. High success rate of model development of brain tumor were established using cell suspensions directly derived from fresh patient brain tumors indicating the important role of stromal cells in tumor formation [11]

Methods

Results

Discussion

Conclusion