Development of Chitosan Silver Nanocomposites: Its Characteristic Study and Toxicity Effect against 3T3-L1 Cell Line

Jesteena Johney,R Ragunathan,S Radhai Sri

doi:10.22207/jpam.16.1.47

Abstract

Nanocomposite from the natural source is opened a wide area for the researchers to find a natural remedy to replace the chemicals or harmful products in all the fields of agricultural, food and medical fields. Here the biopolymer (chitosan) was extracted from the two white rot fungi of Pleurotus floridanus and Pleurotus djamor, and biologically synthesized with 1mM AgNO3 solution. Synthesized chitosan nanocomposite was characterized with UV-Visible study, FTIR, FESEM, XRD, EDAS for the confirmation based upon the peaks, functional group, crystalline nature, size, morphology and the percentage of elements respectively. Toxicity study was carried out using 3T3 L1 (Mouse embryo fibroblast Cell Line) normal Cell Line to find out the cytotoxicity effect of the chitosan nanocomposite and found that the nanocomposites were non toxic to the Cell Line.

Highlights

From the middle of 20th century, the world’s population has engaged to find out a better remedy to treat various diseases caused by microorganisms
Synthesized chitosan nanocomposite was characterized with UV-Visible study, FTIR, FESEM, XRD, EDAS for the confirmation based upon the peaks, functional group, crystalline nature, size, morphology and the percentage of elements respectively
Silver nanocomposite plasmon peak was observed at 460nm (Fig. 2), which indicated the presence of silver nanocomposite

Summary

Introduction

From the middle of 20th century, the world’s population has engaged to find out a better remedy to treat various diseases caused by microorganisms. Nanoparticles are commonly established in the perspective of nanotechnology and Nanoscience is a unit of scientific measurement from the nanometre of 1 – 100nm in size.[3] In current occasion, metallic nanoparticles are showing unique properties and activities such as physical, biological and chemical synthesising. This have more subject of extensive research in recent years.[4,5]

Methods

Results

Conclusion