Abstract
The investigation of the formation of cell wall appendages inAcanthosphaera by means of light and electron microscopy and by the use of dyes which interfere with microfibril assembly resulted in several observations which are helpful to an understanding of the formation of normal cell walls. The barbs are built up in the ER, pass through the Golgi apparatus, and are extruded exocytotically after cytokinesis, a remarkable example of the secretion of a structured product. Each cellulose microfibril in a spike develops in a distinct pit of the plasmalemma. The pits are aggregated in a pit field, generating one spike, and are closely adjacent to a basal vesicle which might have morphogenetic and/or regulatory functions. The pits are the site of cellulose synthesis; here the plasmalemma is conspicuously thickened. As shown directly and by the application of Calcofluor white and Congo red, the microfibrils assemble at a certain distance from the plasma membrane,i.e. cellulose synthesis and microfibril assembly are separated by a gap. It is discussed whether single glucan chains or small bundles of them are released from the plasmalemma. The elongation rate of the spikes indicates that about 1000 glycosidic linkages per glucan chain per minute are formed.
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