Abstract
BackgroundSide population (SP) refers to a group of cells, which is capable to efflux Hoechst 33342, a DNA-binding dye. SP cells exist both in normal and tumor tissues. SP abundance is used as an indicator for disease prognosis and drug screening in some studies. Our study concentrates on the factors influencing SP analysis. Materials and methodsIn this study, we aimed at understanding the multiple factors involved in SP analysis including Hoechst 33342 staining and cell culture. RPMI-8226, a high SP percentage (SP%) human myeloma cell line was employed here. ResultsThe results showed that SP% was subject to staining conditions including: viable cell proportion, dye concentration, staining cell density, and incubation duration. SP% was maximum on day one after passage, while decreased steadily over time. This study shows that staining conditions, culture method and culture duration can significantly affect SP%. In this case, that there was a relation between culture duration and SP%. ConclusionThis indicate that there were different factors effecting cell proliferation in cell culture. Maintaining these factors consistently is essential in SP research.
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