Abstract
In this work, the CdSe–ZnO flower-rod core-shell structure (CSZFRs) was prepared by ion-exchange method. The surface of CSZFRs was modified by 3-mercaptopropionic acid (MPA), and then the DNA probe was immobilized on the surface via chemical bond between -NH2 of DNA probe and -COOH of MPA. Finally, the target norovirous (NV) RNA was combined with the probe according to the principle of complementary base pairing, resulting in a decrease of the photocurrent. The results show that the absorbance spectrum of visible light is enhanced for CSZFRs compared with pure ZnO. Under visible light irradiation, the photocurrent of CSZFRs is up to 0.1 mA, which can improve the sensitivity of the photoelectrochemical (PEC) biosensor. In the measurement range of 0–5.10 nM, the measured concentrations (c) have a good linear relationship with the output photocurrent of the biosensor. The linear regression equation is expressed as I = 0.03256 − 0.0033c (R2 = 0.99, S/N = 3) with a detection limit of 0.50 nM. Therefore, this work realizes a rapid and sensitive method for the detection of NV RNA.
Highlights
Norovirus (NV), one genus of the family of Caliciviridae, is one of the main pathogens causing nonbacterial gastroenteritis in humans
The narrow bandgap CdSe quantum dots (QDs) can overcome the defect of lager bandgap ZnO to absorb the visible region of solar spectrum and enhance the utilization of solar energy
Under visible light irradiation, the photogenerated electrons can transfer from valence band (VB) of CdSe to conduction band (CB), and transfer to CB of ZnO, transfer to the surface of Indium doped tin oxide (ITO) glass to generate photocurrent
Summary
Norovirus (NV), one genus of the family of Caliciviridae, is one of the main pathogens causing nonbacterial gastroenteritis in humans. Nano ZnO has been widely used in the field of biosensors due to the advantages of high electron mobility, stable chemical properties, and good biocompatibility [9]. Because of their high electron transport rate and adsorption surface activity, ZnO nanorods and nanoflowers are used to improve the signal transmission rate and enhance the immobilizing effect of the biological molecules in PEC biosensor [10,11]. It shows a high sensitivity for the low concentration of NV RNA It has great advantages like convenient use, simple equipment, and fast response. The proposed PEC biosensor exhibits broad prospects and potential applications to be explored in biological detection and analysis respect
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