Abstract
: More recently, enzyme-based biofuel cells have attracted special attention in different areas. The alcohol dehydrogenase (ADH) due to its cheap and easily available substrate (ethanol) is one of the dehydrogenase enzymes that has been used on researches extensively. This paper explains procurement and performance of bioanode based ADH. In present paper, we used multi-walled carbon nanotubes (MWCNTs) and polyamidoamine dendrimer (PAMAM) on poly methylene green (PMG) modified carbon cloth to fabricate bioanode for an ethanol biofuel cell. The cyclic voltammetry experiments have used to study the performance of the immobilized ADH in presence of β-nicotinamide adenine dinucleotide (NAD+) and ethanol as cofactor and substrate respectively. Also, the kinetic parameter values like kcat, KMS, and k were calculated using analytical equations based on results obtained from cyclic voltammetry. The maximum anodic current of 153.37 µA obtained from the modified bioanode at a concentration of 300 mM ethanol. The kinetic parameter values kcat, KM, and k (rate constant) for enzyme-substrate reaction calculated 1.6 ± 0.1 S-1, 4.4 mM and 1.9 × 105 M-1S-1 respectively. The results showed that the composition of PAMAM and MWCNTs provides a good electronic communication for activity of ADH based on PMG/PAMAM/MWCNTs.
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