Abstract

In this study, inulin (INU) extracted from burdock root was utilized as a new film base material and combined with chitosan (CHI) to prepare composite films. Oregano and thyme essential oils (OT) were incorporated into the INU-CHI film to confer the films with bioactivities. The physical and optical properties as well as antioxidant and antimicrobial activities of the films were evaluated. INU film alone showed poor physical properties. In contrast, the compatibility of INU and CHI demonstrated by the changes in attenuated total reflectance-Fourier transformation infrared spectrum of the INU-CHI film increased tensile strength and elongation at break of the INU film by 8.2- and 3.9-fold, respectively. In addition, water vapor permeability, water solubility, and moisture content of the films decreased proportionally with increasing OT concentration in the INU-CHI film. Incorporation of OT also increased the opacity of a and b values and decreased the L value of the INU-CHI films. All INU-CHI films containing OT exhibited antioxidant and antimicrobial properties. Particularly, the INU-CHI film with 2.0% OT exhibited the highest 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), 2,2-diphenyl-1-picrylhydrazyl radical scavenging, and antimicrobial activities against four pathogens. Thus, the INU-CHI film containing OT developed in this study might be utilized as an active packaging material in the food industry.

Highlights

  • Inulin (INU) is a polysaccharide with a β-D-fructose chain linked by (2 → 1) glycosidic bonds and one terminal α-D-glucopyranose molecule bound to a fructose chain through a (1 ↔ 2) glycosidic linkage

  • INU extracted from burdock roots has not been studied as an edible packaging material

  • Means ± S.D. a–f: any means in the same column followed by different letters are significantly (p < 0.05) different by Duncan’s multiple range test

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Summary

Introduction

Inulin (INU) is a polysaccharide with a β-D-fructose chain linked by (2 → 1) glycosidic bonds and one terminal α-D-glucopyranose molecule bound to a fructose chain through a (1 ↔ 2) glycosidic linkage. INU is mainly found in artichoke tubers, chicory, yacon, and burdock roots. Based on the physicochemical characteristics of INU, such as its film-forming capacity, it can be used with other polymers to prepare blend films. INU extracted from burdock roots has not been studied as an edible packaging material. Antimicrobial activities, low oxygen permeability, and good mechanical properties, CHI has been combined with other hydrocolloids such as starch, gelatin, and alginate to produce blended or multi-layered films [7,8,9]. Antimicrobial and/or antioxidant substances have been incorporated into active packaging films [eafi1scl0stmie]v.nsiAOtt[ii1naer0elsti]gm.o[a1inOil1cso]rr.eofeogCbsraisaanertlnvohataeiencasrpdlsor/eolen,oipltrtaih(aOraylanmEtoti)iiooolnalx(,nOidoγdEfa-t)tneehatrdypnsimbidunlebeetshnetfyseaisl,mnmeacnesentseidbashlespaeco-anvciulyetism(abTeleeEoone)ifnela(traihTnereEectio)wrtrahpoarenoeortmtfiamwttaehoiidcnerooimbfnbittooihoasaeltcatmaccionvtoimedvsetmcaocpnoomatnmicoplkyxmoaiuudgonsainnednldgyst fTuaocEsuteinvd[1dite2isie,n1sse3[On]1.tE1iA]a.alCnocdaoilrmsTvfEaboci.rnrCotahlat,ierotvhpnayrcmeorpofoallOr,isaγEta-itoaemnrnpadoijfnoTeerEndceiob,omlarenpcfidoalnpmrev-csnaytcbmrieoncelanOuaeEnsae,drweotfthhhtiyhelememitorhalayinnmhtbaiomsiol aibicscertetoihvnbeeiarcmelopaomonsptrdtoaeaubdnnutdntioosdxafsiondhutoaninwndt sinynOeErgainstdicToEr.aCdadrivtaivceroelfifsecatsmoanjoarnctoimmpicornoebniatlinacOtivEi,twy h[1il4e,1t5h]y.mol is the most abundant in TE [12,13]. IIoonncchhrroommaattooggrraamm ooff iinnuulliin (INNU) extracted from burdock root

PPhhyysical Properties of the Films
Optical Properties
Materials
Extraction of INU
Determination of DP of INU Extracted from Burdock Roots
Film Preparation
Physical Properties Measurement
Optical Properties of the Films
ATR-FTIR Spectroscopy
Antioxidant Activities
3.10. Antimicrobial Activities
Findings
Conclusions
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