Abstract
Selenium nanostructures are characterized with high activity and antibacterial properties. Selenium nanoparticles, received by the biotechnological method using microorganisms are not toxic and free from admixtures of other toxic elements. For getting selenium nanostructures, there was set a problem of development of the biotransfrormation technology of inorganic selenium by the culture Lactobacillus acdophilus, strain 412/307 from the museum of the Department of Biochemistry, Microbiology and Physiology of Nutrition Odessa National Academy of Food Technologies (Ukraine). Selenium nanostructures are the side product in the process of selenocysteine formation. There was realized the study of the influence of the sodium selenite concentration on the growth speed and generation duration of lactobacteria on MRS medium for determining the main technological parameters. There was determined the quantitative content of accumulated selenium at different initial concentrations of sodium selenite in the medium. The important part of the elaborated technology is destruction of cellular walls of microorganisms for removal selenium nanoparticles. There was studied the influence of different physical-chemical factors of cells’ destruction. There was offered to use the combined influence of saline acid, freezing and microwave field for destructing cellular walls of lactobacteria biomasses. There is presented the scheme of the technology of getting “Nanoselenium” and given physical-chemical parameters of the received product.
Highlights
Selenium it is an essential microelement that plays an important role in physical processes in the human organism
Literary data testify that the biological method of getting nanoparticles of elements can be realized using microorganisms, especially in the process of their cultivation in mediums with the content of inorganic forms of studied elements [2,3,4]
MRS broth was chosen as a medium for lactobacteria cultivation
Summary
Selenium it is an essential microelement that plays an important role in physical processes in the human organism It is included in the composition of many proteins and enzymes. Literary data testify that the biological method of getting nanoparticles of elements can be realized using microorganisms, especially in the process of their cultivation in mediums with the content of inorganic forms of studied elements [2,3,4]. This method of nanoparticles creation has a (2018), «EUREKA: Life Sciences» Number 1 series of advantages, namely the short time of cultivation of microorganisms and impossibility of forming side toxic products [5]
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