Abstract
The wide application of nanomaterials allows us to reconsider common methods in various fields of science. Nanomaterials have a great potential of application due to their small size and selectivity in cellular mechanisms. Here we use three types of nanoparticles with different morphology: magnetic nanoparticles (MNPs), halloysite nanotubes (HNTs) and graphene oxide (GO). The purpose of the study was to create surfaces with unique nanotopography. Characterization of nanomaterials was carried out by a combination of atomic force microscopy (AFM) imaging and dynamic light scattering (DLS) technique. Method of dip-coating was used to produce nanotopography on surfaces, observed then by dark field microscopy (DFM) imaging. The results showed that the mean size of MNPs, HNTs, and GO was 141.7±2.8, 310.5±4.2, and 1944.2±89.1 nm, respectively. Surface characterization by DFM imaging showed different types of roughness made by nanoparticles, and spectrum intensity in VNIR spectra. The obtained data helps us to conduct the investigation of the influence of surface nanotopography on cell fate.
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