Development of banana in vitro from male bud culture supplemented with some concentration of sucrose and benzyladenine

Abstract

Conventional techniques of propagating banana plants with corms require a relatively long time (10-18 months) and the amount produced is limited due to deforestation and industrialization. So production and supply of quality products are becoming a great challenge. In addition, there is a need to develop climate-resilient crop to face the consequences of global warming in the near future. Plant tissue culture is a proven technique for producing banana seeds in large quantities, uniformly and in a short time to support good quality banana seeds. The banana flower meristem can be a potential explant. The banana flower meristem offers the opportunity to regenerate plants with agronomic characteristics and results that can be controlled. This study aimed to regenerate banana flowers in vitro with different sucrose and BA (Benzyladenine) concentrations. The study used a Completely Randomized Design (CRD), two factorials sucrose concentration with 4 levels (20 g/L, 25 g/L, 30 g/L, and 35 g/L) and BA concentration with 4 levels (2 ppm, 4 ppm, 6 ppm and 8 ppm). The results showed that the combination of BA and sucrose concentration had not directly induced organogenesis in banana flower explants. Growth and development of banana flower explants maximally form Cauliflower-Like Bodies.