Abstract
BackgroundThe present study was carried out to prepare multi-herbal combination via comparing antioxidant activity and polyphenolic composition of five medicinal plant extracts of Punica granatum L., Putranjiva roxburghii Wall., Swertia chirata Buch.-Ham., Tinospora cordifolia (Willd.) Miers and Trigonella corniculata L.MethodsThe herbs were individually evaluated using in vitro antioxidant assays and analyzed by HPLC-PDA. The resultant data was examined using principal component analysis (PCA). Further, herbal combination was prepared on the basis of PCA.ResultsThe PCA divided the plants into three groups. The leading or primary group contained P. granatum and P. roxburghii with the highest antioxidant activity strongly correlated with high amount of kaempferol. S. chirata was acknowledged as nourisher herb in one and T. cordifolia and T. corniculata were identified as stimulator herbs in other group. The herbal combination exhibited high antioxidant activity as compared to the individual plants. The combination revealed good antiproliferative efficacy against hepatocellular carcinoma (HepG2) cells with IC50 of 75.864 μg/ml.ConclusionsThe activity observed in vitro with HepG2 cells suggests that the herbal combination can provide therapeutic activity in vivo in future. The study may provide information regarding precise preparation of multi-herbal formulations using PCA as a tool in pharmaceutical industries.
Highlights
The present study was carried out to prepare multi-herbal combination via comparing antioxidant activity and polyphenolic composition of five medicinal plant extracts of Punica granatum L., Putranjiva roxburghii Wall., Swertia chirata Buch.-Ham., Tinospora cordifolia (Willd.) Miers and Trigonella corniculata L
Phytotherapeutic management of pathological conditions encompasses combinatorial intervention of multiple bioactive constituents manifesting multi-target strategy [1]. The polyphenols from these strategies represent an important diverse cluster of phytochemicals primarily contributing to the remedial measures and prevention of many diseases. These phytoconstituents are usually hydrophobic in nature and their hydrophobicity is intermediate between vitamin C and vitamin E [2]
In order to get a more extensive depiction, we examined the antioxidant capacities and polyphenolic composition(s) of different eco-solvent extracted medicinal plant extracts supported by the HPLC-photo diode array (PDA) analysis
Summary
The present study was carried out to prepare multi-herbal combination via comparing antioxidant activity and polyphenolic composition of five medicinal plant extracts of Punica granatum L., Putranjiva roxburghii Wall., Swertia chirata Buch.-Ham., Tinospora cordifolia (Willd.) Miers and Trigonella corniculata L. Phytotherapeutic management of pathological conditions encompasses combinatorial intervention of multiple bioactive constituents manifesting multi-target strategy [1]. The polyphenols from these strategies represent an important diverse cluster of phytochemicals primarily contributing to the remedial measures and prevention of many diseases. These phytoconstituents are usually hydrophobic in nature and their hydrophobicity is intermediate between vitamin C (highly hydrophilic) and vitamin E (highly hydrophobic) [2]. Water at elevated temperature under pressure is comparable to organic solvent extraction [6]. The elevated temperature reduces the dielectric constant of water making it competent to dissolve even hydrophobic organics such as polycyclic aromatic hydrocarbons and polychlorinated biphenyl analogs. In this report we have examined the activity of aqueous extracts of several selected herbs both alone as well as in combination
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