Abstract
Aptamers are short single stranded DNA or RNA selected through systematic evolution of ligands by exponential enrichment (SELEX), which can bind to target molecules with high affinity and high specificity. Proteins are vitally functional macromolecules. Many SELEX techniques have been developed for screening of aptamers against proteins. In recent years, in order to obtain aptamers with high affinity and specificity, many SELEX methods have been developed to enhance selection efficiency, reduce cost, and improve aptamer properties. Affinity characterization is also the key procedure in aptamer selection, which demonstrates whether the obtained aptamer could satisfy the application requirement. In this review, the progress in aptamer screening for protein targets since 2016 was summarized, and the optimization methods of nucleic acid library, improvement and development of screening methods, and application of aptamers against proteins were mainly emphasized.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.