Abstract
To prevent confusing Dioscorea nipponica (DN), an Oriental medicine, with Dioscorea quinquelobata (DQ) and Dioscorea septemloba (DS), a simple and accurate quantitative analysis method using HPLC combined with ultraviolet (UV) detection was developed and verified with UPLC-QTOF/MS through identification of five saponin glycosides: protodioscin (1), protogracillin (2), pseudoprotodioscin (3), dioscin (4), and gracillin (5). The newly developed analysis method showed sufficient reproducibility (<1.91%) and accuracy (92.1%–102.6%) and was able to identify DN based on the presence of compound 3 (13.821 ± 0.037 mg/mL) and the absence of 5. Compound 1, which is present in DN at a relatively high level (159.983 ± 0.064 mg/mL), was also an important marker for identification. Among the three species, DN showed the strongest activation of apoptotic signaling in osteosarcoma cells, while the four compounds detected in DN showed IC50 values of 6.43 (1), 10.61 (2), 10.48 (3), and 6.90 (4). In conclusion, the strong inhibitory effect of DN against osteosarcoma was confirmed to be associated with 1 and 4, which is also related to the quantitative results. Therefore, the results of this study might provide important information for quality control related to Oriental medicine.
Highlights
Among the various Dioscorea families, Dioscorea nipponica (DN) is a wild perennial species that is widely distributed in the Korean peninsula, Japan, and China, along with Dioscorea tokoro, Dioscorea japonica, Dioscorea tenuipes, Dioscorea quinquelobata (DQ), and Dioscorea septemloba (DS)
The HPLC analysis method developed above was validated based on linearity (r2 > 0.999), accuracy (92.1%–106.2%), precision (
A UHPLC-Quadrupole time-of-flight MS (QTOF/MS) analysis confirmed that the five steroidal saponins quantified by HPLC-UV were present in the samples, and their structures were predicted based on the loss of one or two sugars, which were visible in the MS/MS
Summary
Among the various Dioscorea families, Dioscorea nipponica (DN) is a wild perennial species that is widely distributed in the Korean peninsula, Japan, and China, along with Dioscorea tokoro, Dioscorea japonica, Dioscorea tenuipes, Dioscorea quinquelobata (DQ), and Dioscorea septemloba (DS). This medicine has traditionally been prescribed for the treatment of rheumatism, asthma, and bronchitis; alleviating pain; and improving blood circulation. The origin of the species specified in the standard medicines of North Korea [1] and China [2] is designated as DN. In China, which is a major producer of DN, approximately 49 species of the genus Dioscorea are found, and two of these species, DN and Dioscorea panthaica, are systematically managed by the Chinese. Pharmacopeia Committee (CHD) as raw medicine materials [3].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
