Abstract
A simple and selective spectrofluorimetric method for the detection of chlortetracycline (CTC) was studied. In pH7.4 buffer medium l-tryptophan (l-Trp), applied as the fluorescence probe, interacted with CTC resulting in fluorescence quenching of the probe. CTC was detected with maximum excitation and emission wavelengths at λex /λem =275/350nm. Notably, quenching of fluorescence intensities was positively proportional to the CTC concentration over the range of 0.65-30μmol L-1 and the limit of detection was 0.2μmol L-1 . Effect of temperature shown in Stern-Volmer plots, absorption spectra and fluorescence lifetime determination, indicated that fluorescence quenching of l-Trp by CTC was mainly by static quenching. The proposed study used practical samples analysis satisfactorily.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Luminescence : the journal of biological and chemical luminescence
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
