Abstract
A dimethylferrocene-mediated enzyme electrode for glucose (Cass et al., 1984) was evaluated for application to microbial fermentation monitoring and control. The deficiencies revealed - insufficient stability, progressive increase in response time and progressive decrease in linear range - were investigated using electrochemical and radiochemical techniques. The enzyme immobilization was improved with a novel procedure using periodate-oxidized glucose oxidase and an alky lamine electrode coating. An in-situ probe and a computer-controlled analytical system were developed for fermentation monitoring. The glucose concentration of an Escherichia coli batch culture was successfully monitored on line using this analytical system.
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