Abstract
Dexketoprofen (D-KTP), tramadol (TMD), and haloperidol (HLP), two analgesic agents and an adjuvant, respectively, were analysed simultaneously in 0.9% NaCl and 5% glucose by use of a new, rapid LC–DAD method. Chromatographic separation was achieved on a C18 column with 50:50 (v/v) acetonitrile–sodium dihydrogen phosphate (pH 4.10; 0.1 M) as mobile phase at a flow rate of 1 mL min−1. Ultraviolet diode-array detection was used. The absorbance of the eluate was monitored at 210 nm. The retention times of the three compounds were 2.0, 4.0, and 8.3 min for TMD, HLP, and D-KTP, respectively; the total run time was 9 min. The method was validated for linearity, accuracy, reproducibility, and limits of quantification and detection. Calibration functions were established between 100.0 and 225.0 mg L−1 for D-KTF, between 200.0 and 450.0 mg L−1 for TMD, and between 2.0 and 4.5 mg L−1 for HLP. Recoveries were >98% with precision less than 2.2% for the three drugs prepared in both 0.9% NaCl and 5% glucose. The method was successfully used to evaluate the chemical stability of this drug mixture at the usual concentration used in patient-controlled analgesia. Samples were prepared at 2.5 mg mL−1 D-KTF, 5.0 mg mL−1 TMD, and 0.05 mg mL−1 HLP in 0.9% NaCl and in 5% glucose. Stability was assumed if the loss was less than 10% of the initial concentration. Five different storage conditions were tested for 30 days and the drug mixture was stable under all these. Stability was independent of medium, 0.9% NaCl or 5% glucose. The best results were obtained for the drug mixture stored at 4 °C in darkness.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.