Abstract
This study adopted a microelectromechanical fabrication process to design a chip integrated with electroosmotic flow and dielectrophoresis force for single cell lysis. Human histiocytic lymphoma U937 cells were driven rapidly by electroosmotic flow and precisely moved to a specific area for cell lysis. By varying the frequency of AC power, 15 V AC at 1 MHz of frequency configuration achieved 100% cell lysing at the specific area. The integrated chip could successfully manipulate single cells to a specific position and lysis. The overall successful rate of cell tracking, positioning, and cell lysis is 80%. The average speed of cell driving was 17.74 μm/s. This technique will be developed for DNA extraction in biomolecular detection. It can simplify pre-treatment procedures for biotechnological analysis of samples.
Highlights
Cell membrane perforation and cell lysis are commonly used in cell engineering with applications in transgenic cells to obtain DNA
Sensors 2012, 12 selects the most special cell as sample, so we develop the integrated chip for automatic tracking and positioning manipulation of single cell lysis
The results showed that when a cell receives downward pressure from a probe tip at a distance of 1–2 μm, this is sufficient to complete cell lysis
Summary
Cell membrane perforation and cell lysis are commonly used in cell engineering with applications in transgenic cells to obtain DNA. Cell lysis results in the destruction of cell membranes by mechanical, physical, chemical, and electrical methods. Cell lysis directly occurring on a chip can simplify the pre-treatment procedures for biotechnological analysis of samples. Sensors 2012, 12 selects the most special cell as sample, so we develop the integrated chip for automatic tracking and positioning manipulation of single cell lysis. The probe tip was used to carry out lysis of a living cell. The results showed that when a cell receives downward pressure from a probe tip at a distance of 1–2 μm, this is sufficient to complete cell lysis. Youn et al created compression and DNA storage areas in the microfluidic channel designed to cause cell deformation and rupture by extrusion [3]
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