Development of an inexpensive controlled rate freezer for semen cryopreservation

Abstract

It is common for assisted reproductive technology (ART) laboratories to cryopreserve semen as backup, or to pool semen for ART procedures. Currently, labs generally either hang prepared semen in nitrogen mist or use an electronic, controlled rate freezing device (ECRF). The first method is inexpensive, but yields highly variable results, the second method requires equipment which is expensive to purchase and operate. The objective of the present study was to compare a new, inexpensive, mechanical controlled rate freezer (MCRF) developed in this laboratory to the two standard methods.