Abstract

From 1993 to 1996, fish assemblage data were collected from 309 wadeable streams in the U.S. Mid-Atlantic Highlands region as part of the U.S. Environmental Protection Agency's Environmental Monitoring and Assessment Program. Stream sites were selected with a probabilistic sampling design that allowed regional estimates of stream condition. We examined responses of 58 fish assemblage metrics to physical, chemical, and landscape indicators of disturbance. Uni- variate and multivariate analyses of relationships among fish metrics, habitat integrity, and an- thropogenic disturbance were used to develop a fish index of biotic integrity (IBI) for assessing stream condition in the entire region. Of 58 candidate metrics 9 were selected and scored contin- uously from 0 to 10; the resulting IBI was scaled so that it ranged from 0 to 100. Regional estimates of stream conditions showed that 27% of the stream length in the Mid-Atlantic Highlands had fish assemblages in good or excellent ecological condition. Of the total wadeable perennial stream length in the region 38% was fair and 14% was poor. There were insufficient data to calculate IBIs for 21% of the wadeable stream length in the Mid-Atlantic Highlands; all of these streams were small (watershed area # 2k m 2) and lacked sufficient sample size (,10 individuals) to calculate

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