Abstract
Stone fruits are well known for their high nutritional value. Therefore, in horticulture, the micropropagation of suitable rootstocks is vital for their cultivation. The aim of the present study was to improve the micropropagation protocol of Myrobalan 29C (Prunus cerasifera Ehrh.) rootstock using shoot-tip culture. Murashige and Skoog (MS) basal medium containing 2 mg L-1 6-benzylaminopurine (BAP) and 0.05 mg L-1 gibberellic acid (GA3) resulted in the highest number (14.3) of multiple shoots. However, a greater shoot length (2.0 cm) was attained when GA3 was excluded from the MS medium and the concentration of BAP was reduced to 1 mg L-1. Root induction was best in ½MS medium containing 0.5 mg L-1 indole-3-butyric acid (IBA), 0.5 mg L-1 α-naphthaleneacetic acid, and 10 mL L-1 (≈13 mg L-1 Fe) ethylenediamine di-2-hydroxyphenyl acetate ferric with 7.0 roots per explant. On the other hand, the longest root (12.5 cm) was obtained from increased concentration to 1 mg L-1 of IBA. The establishment of a well-defined micropropagation protocol will lead to further biotechnological improvement of this crop.
Highlights
Horticultural plants are nutritious, tasty, and healthy for humans (Sahin et al, 2002; Ozturk et al, 2009; Gündüz and Özbay, 2018)
Positive and noteworthy results for shoot multiplication and root induction were obtained from the in vitro clonal propagation of Myrobalan 29C clone rootstock
The Murashige and Skoog (MS) medium containing 1 and 2 mg L–1 BAP gave the best results for the formation of multiple shoots per explant
Summary
Horticultural plants are nutritious, tasty, and healthy for humans (Sahin et al, 2002; Ozturk et al, 2009; Gündüz and Özbay, 2018). Consumers prefer stone fruits, like plums and apricots, for their delicious taste and higher nutritional content. Myrobalan (Prunus cerasifera Ehrh.) probably originated in the Caucasus and comprises many varieties with different characteristics (Güleryüz and Ercişli, 1995). It is suitable for all soil types and adapts well to dry soils and heavy soils with low permeability. Myrobalan 29C is the selected rootstock for plum and apricot due to its resistance to root-knot nematodes (Meloidogyne). The apricots grafted on Myrobalan rootstocks exhibit moderate vigor and live up to 25–30 years (Gönülşen et al, 1987; Dimitrova, 1988; Özçağıran et al, 2005). The hybrid produced in this method inherits all the properties such as adaptation ability, rapid
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