Abstract
A multi-mycotoxin chromatographic method was developed and validated for the simultaneous quantitation of aflatoxins (AFB1, AFB2, AFG1 and AFG2), ochratoxin A (OTA), zearalenone (ZON), deoxynivalenol (DON), nivalenol (NIV), diacetoxyscirpenol (DAS), fumonisins (FB1, FB2 and FB3), T-2 toxin (T-2) and HT-2 toxin (HT-2) in feed. The three most popular sample preparation techniques for determination of mycotoxins have been evaluated, and the method with highest recoveries was selected and optimized. This modified QuEChERS (quick, easy, cheap, effective, rugged and safe) approach was based on the extraction with acetonitrile, salting-out and cleanup with lipid removal. A reconstitution process in methanol/water was used to improve the MS responses and then the extracts were analyzed by LC-MS/MS. In this method, the recovery range is 70–100% for DON, DAS, FB1, FB2, FB3, HT-2, T-2, OTA, ZON, AFG1, AFG2, AFB1 and AFB2 and 55% for NIV in the spike range of 2–80 µg/kg. Method robustness was determined with acceptable z-scores in proficiency tests and validation experiments.
Highlights
Mycotoxins are the most common contaminants in agricultural crops produced by several species of mold and fungi
Key Contribution: This study describes an improved analytical method for quantitation of common mycotoxins with acceptable recoveries in different feed products
Creating an accurate and fast analytical method to quantify the contamination levels of mycotoxins plays a vital role in food and feed safety assessment risks
Summary
Mycotoxins are the most common contaminants in agricultural crops produced by several species of mold and fungi. Maturity, harvest, storage and processing of food and animal feed products, the fungus produces mycotoxins and other secondary metabolites [1]. These mycotoxin-contaminated food and feed threaten human and animal health even at very low concentration [2]. The presence of mycotoxins in consuming animal products such as milk and meat are a significant safety concern as well [5,6]. Creating an accurate and fast analytical method to quantify the contamination levels of mycotoxins plays a vital role in food and feed safety assessment risks
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