Abstract
This paper presents an impedimetric immunobiosensor for detecting the impedance signals of various concentrations of carcinoembryonic antigen (CEA), a type of protein molecule which is typically associated with certain tumors. The biosensor was developed by parallel electrodes and improved sandwich immunoassay, and used specific binding of antibodies to change the impedance signals of various CEA concentrations. We observed an obvious difference in impedance signals after 13min, and a better linearity (a steep slope between impedance and CEA concentration) at 100Hz. Finally, when the concentration of the AuNPs-anti-CEA antibody was diluted 30 for times, the detection limit for CEA concentration was 1ng/mL with 1μg/mL of anti-CEA antibody. Therefore, the developed biosensor has advantages including smaller sample volume (25μL), rapid quantitative measurement (10min), and smaller detectable concentrations (1ng/mL).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
