Abstract
A colloidal gold (ICS) test was developed for rapid detection of zearalenone (ZEN) in wheat samples. The mAb against ZEN was prepared in our laboratory and labelled with colloidal gold as a probe for the ICS test. The conditions were optimized and 30 nm colloidal gold nanoparticles were chosen for optimal performance. Millipore 135 was chosen as the NC membrane for its level of sensitivity. The optimum amount of coated antigen ZEN-OVA and anti-ZEN mAb was 0.5 mg/mL and 8 μg/mL, respectively. The ICS test, which has a detection limit of 15 ng/mL for ZEN, could be completed in 5 min. Analysis of ZEN in 202 wheat samples over three consecutive years revealed that data obtained from the ICS test were in a good agreement with LC-MS/MS data. This result demonstrated that the ICS test could be used as a qualitative tool to screen on-site for ZEN.
Highlights
Mycotoxins are known as poisonous metabolites of fungi that exist on farmland or in the process of producing, transporting, handling or storing farm commodities and foodstuff [1,2]
Bovine serum albumin (BSA), sodium dodecyl benzenesulfonate (SDBS), goat anti-mouse IgG, mycose, polyethylene glycol (PEG, MW = 20,000), polyvinylpyrrolidone (PVP), sucrose, Tween-20 were purchased from the Sino-American Biotechnology Co. (Shanghai, China)
Wheat samples were collected from several regions of Jiangsu province, southeast China, that are commonly heavily infected with Fusarium (S1 Table); The survey was conducted over
Summary
Mycotoxins are known as poisonous metabolites of fungi that exist on farmland or in the process of producing, transporting, handling or storing farm commodities and foodstuff [1,2]. Up to 25% of grain crops are contaminated with mycotoxins, which results in substantial economic losses all around the world [3]. Considering the substantial risks of public health and economic, it has received much attention among scientists who attempt to detect mycotoxins in foodstuff and fodder in recent years [5]. As a nonsteroidal mycotoxin which possesses estrogen-like activity, ZEN has been observed to be related to pubertas praecox, endometrial hyperplasia, endometrial neoplasms as well as carcinoma of uterine cervix [6].
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