Development of an immunochromatographic strip test for rapid detection of ciprofloxacin in milk samples.

Liqiang Liu,Steven Suryoprabowo,Liju Luo,Chuanlai Xu,Juan Peng,Hua Kuang

doi:10.3390/s140916785

Abstract

A rapid, simple, and sensitive immunochromatographic test strip has been developed for testing residues of ciprofloxacin (CIP). A specific and sensitive monoclonal antibody (mAb) for CIP was generated by immunizing BALB/c mice with well-characterized CIP-Keyhole limpet haemocyanin. Under the optimized conditions, the cut-off limits of test strips for CIP were found to be 5 ng/mL in phosphate-buffered saline and 2.5 ng/mL in milk samples. Each test can be evaluated within 3 min. The cross-reactivities of the CIP test strip to enrofloxacin (ENR), norfloxacin (NOR), nadifloxacin (NDF), danofloxacin (DANO), pefloxacin (PEX), lomefloxacin (LOME), enoxacin (ENO), and sarafloxacin (SAR) were 71.4%, 71.4%, 66%, 50%, 33%, 20%, 12.5%, and 6.25%, respectively. The data indicate that the method is sensitive, specific, and has the advantages of simplicity and speed, therefore, this test strip is a useful screening method for the detection of CIP residues in milk samples.

Highlights

Fluoroquinolones (FQs) are the most important group of synthetic antimicrobials, which are widely used in veterinary and aquatic medicine
A sensitive colloidal gold-based lateral-flow immunochromatography assay was developed for the rapid detection of CIP in milk samples
A highly sensitive and specific anti-CIP antibody was obtained from BALB/c mice with CIP-keyhole limpet hemocyanin (KLH) conjugates

Summary

Introduction

Fluoroquinolones (FQs) are the most important group of synthetic antimicrobials, which are widely used in veterinary and aquatic medicine. The presence of antimicrobial drug residues in food carries potential risk by selection of resistant pathogenic organisms and causes adverse effects on intestinal microflora, which could decrease the quality of animal products. In the mid-1980s and 1990s, FQs were introduced for human use in Europe and the United States and approved for livestock treatment [3] and quickly become the most commonly prescribed antibiotics. The widespread use and possibility of abuse or misuse of FQs in agriculture and aquaculture has resulted in the potential presence of residues of these compounds in foodstuffs of animal origin, which has given rise to public health concerns over their toxic effects, development of resistant strains of bacteria, and allergic hypersensitivity reactions [8,9,10]

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