Abstract
ABSTRACT Following the steps of antigen synthesis, immunization, cell fusion, ascites preparation and purification, a maduramicin (MD) monoclonal antibody (mAb) was produced. This MD-mAb demonstrated a 50% inhibition concentration value of 3.75 ng/ml, an affinity constant of 3.70 × 1010 l/mol, and the isotype was IgG3. The MD-mAb has no cross-reactivity with other polyether antibiotics. Using this MD-mAb, a gold immunochromatographic assay was developed to detect MD residues in chicken breast and egg samples. For semi-quantitative detection by the naked eye, the visual limit of detection was 5 ng/g in chicken breast, 10 ng/g in egg. Quantitative results can be obtained by a hand-held strip scan reader, with the detection range of 5.11–19.34 ng/g in chicken breast and 6.46–27.87 ng/g in egg. The strip test took 10 min to run in total. This strip assay is suitable for on-site detection of MD residues in chicken breast and egg samples.
Highlights
IntroductionMaduramicin (MD) is a relatively new monoglycoside polyether ionophore antibiotic, which was derived from the Actinomadura rubra in 1983
KEYWORDS Maduramicin; strip test; chicken breast; egg Maduramicin (MD) is a relatively new monoglycoside polyether ionophore antibiotic, which was derived from the Actinomadura rubra in 1983
In this research, based on the preparation of the MD-monoclonal antibody (mAb), we developed a gold immunochromatography assay for detection of MD residues in chicken breast and eggs samples
Summary
Maduramicin (MD) is a relatively new monoglycoside polyether ionophore antibiotic, which was derived from the Actinomadura rubra in 1983. Based on an anti-MD polyclonal antiserum, Wang, Zhang, Murtazina, Eremin, and Shen (2008) developed a fluorescence polarization immunoassay for MD detection in chicken muscle, fat and egg samples All of these immune assay methods have the features of short assay time, simple to operate, cost-effectiveness and being high throughput. With the development of nanotechnology, antibody has been labelled by novel nanomaterials, including nanoparticles, quantum dots and up-conversion materials, to develop immune chromatography Some of these reagents have already been used for medical diagnosis and veterinary drug residues in food (Guo et al, 2015; Kong et al, 2016; Li et al, 2015, 2016; Sharma, Ragavan, Thakur, & Raghavarao, 2015; Wang et al, 2015; Wu et al, 2017; Xing et al, 2015). In this research, based on the preparation of the MD-mAb, we developed a gold immunochromatography assay for detection of MD residues in chicken breast and eggs samples
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