Abstract
An immunoassay method for determination of fluoroquinolones (FQs) residues in water samples has been developed. For this purpose, NHS ester technology was employed to synthesize the immunogen and coating antigen of sarafloxacin (SAR). SDS-PAGE, UV-visible spectra and infrared spectra identification showed that the artificial antigen was conjugated successfully. Based on the square matrix titration, an indirect competitive ELISA (icELISA) method was established. The dynamic range in assay buffer was from 0.048 to 62.4 ng/mL, with LOD and IC 50 value of 0.018 and 2.5 ng/mL, respectively. This assay showed a high cross-reactivity to difloxacin (86.7%), norfloxacin (63.2%), and pefloxacin (33.8%). When applied in authentic tap water, river water and fishing water samples, the recoveries for sarafloxacin, difloxacin, norfloxacin and pefloxacin were 89.5-119.5%, 90.5-121.2%, 92.1-118.6%, and 88.2-116.8%, respectively, with coefficient of variation (CV) values all < 10%. The results suggest this immunoassay can be used for simultaneous detecting sarafloxacin, difloxacin, norfloxacin, and pefloxacin residues in water samples.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
