Abstract
A novel and simple method for detecting 6 zearalenones in animal feed using liquid chromatography coupled with triple quadrupole mass spectrometry (LC-MS/MS) and immunoaffinity columns (IAC) was developed. The chromatographic peaks of the 6 zearalenones were successfully identified by comparing their retention times and mass spectrum with reference standards. The mobile phase was composed of mobile phase A (water) and B (0.5% formic acid in ACN). Method validation was performed with linearity, sensitivity, selectivity, accuracy and precision. The limits of detection (LODs) for the instrument used to study zearalenones ranged from 0.3 to 1.1 μg/kg, and the limits of quantification (LOQs) ranged from 1.0 to 2.2 μg/kg. Average recoveries of the 6 zearalenones ranged from 82.5% to 106.4%. Method replication resulted in intra-day and inter-day peak area variation of <3.8%. The developed method was specific and reliable and is suited for the routine analysis of zearalenones in animal feed.
Highlights
Zearalenone (ZON) is fungal toxin, a harmful substance for feed
The liquid chromatography (LC)-mass spectrometry (MS)/MS method had been developed by using immunoaffinity columns (IAC) was validated to verify that its performance was compatible with the required performance for routine Zearalenone group (ZEN) analysis in animal feeds
The chromatograms of the ZEN indicate the separation of all six compounds in
Summary
Zearalenone (ZON) is fungal toxin, a harmful substance for feed. The worldwide spotlight is shed upon its contamination of human food and animal feed. ZON has α-zearalanol(α-ZAL), β-zearalanol(β-ZAL), α-zearalenol(α-ZOL) and β-zearalenol(β-ZOL), zearalanone(ZAN), which are isomer and metabolite, and these six types are called Zearalenone group (ZEN)[3]. There exists an analysis method for ZON in feeds, but there are no analytical studies on its isomers.
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