Development of an immobilized liposome chromatography method for screening and characterizing α-glucosidase-binding compounds

Abstract

Immobilized liposome chromatography (ILC) is a powerful tool in screening and analyzing membrane-permeable components in natural medicinal herbs. In this study, the stationary phase of a new receptor liposome biomembrane chromatography (RLBC) was employed to screen the active ingredients in seven natural medicinal herbs. As a model system, α-glucosidase was immobilized in porous silica gel by incorporating α-glucosidase into liposome vesicles. Combined with HPLC, this stationary phase can be used to evaluate the interaction of liposome-protein compounds with compounds and drugs. The surface characteristics of the RLBC phase was characterized by Scanning electron microscope (SEM), Fourier transform infrared spectroscopy (FTIR) and Energy dispersive spectrometer (EDS). RLBC was successfully used to screen and analyze permeable compounds in natural medicinal herbs, and screen the extracts from Schisandra chinensis. This method was used to establish that Schisandrin in Schisandra chinensis is an active component. Furthermore, the hypoglycemic effect of Schisandrin was verified in vivo in rats. This study further modeled the relationship between the activity of inhibitor and retention behavior with RLBC with a mathematical equation. RLBC stationary phase combined with HPLC can be used for rapid screening of drug candidates.