Development of an IgY-based lateral flow immunoassay for detection of fumonisin B in maize.

Tien Viet Tran,Ba Van Nguyen,Son Cao Tran,Thao Phuong Thi Nguyen,Chuyen Van Nguyen,Tung Thanh Tran,Binh Nhu Do,Hoa Quang Le

doi:10.12688/f1000research.19643.2

Abstract

Fumonisin is one of the most prevalent mycotoxins in maize, causing substantial economic losses and potential health risks in human and animals. In the present study, in-house polyclonal IgY antibody against fumonisin group B (FB) was applied for the development of a competitive lateral flow immunoassay detecting these mycotoxins in maize grains with the limit of detection of 4000 µg/kg, which corresponds to the maximum residue limit adopted by The International Codex Alimentarius Commission. To this end, factors affecting the test performance including nitrocellulose membrane type, dilution factor of maize homogenates in running buffer, amount of detection conjugate, and incubation time between detection conjugate and samples were optimized. Under the optimal condition (UniSart ®CN140 nitrocellulose membrane, FB 1-BSA immobilized at 1 µg/cm, 1:10 dilution factor, 436 ng of gold nanoparticle conjugate, 30 minutes of incubation), the developed test could detect both FB 1 and FB 2 in maize with limit of detection of 4000 µg/kg, and showed no cross-reactivity to deoxynivalenol, ochratoxin A, aflatoxin B1 and zearalenone. When applied to detect FB 1 and FB 2 in naturally contaminated maize samples, results obtained from the developed assay were in good agreement with those from the high-performance liquid chromatography method. This lateral flow immunoassay is particularly suitable for screening of fumonisins in maize because of its simplicity and cost-effectiveness.

Highlights

Y antibody against fumonisin B1 (FB1) and B2 (FB2) was applied for the development of a competitive lateral flow immunoassay detecting these mycotoxins in maize grains with the limit of detection of 4000 μg/kg, which corresponds to the maximum residue limit adopted by the European Commission
We demonstrated the development of a IgY-based lateral flow immunoassay (LFIA) for simple and cost-effective screening of total fumonisins in raw maize with limit of detection (LOD) equal to the maximum residue limit (MRL) of 4000 μg/kg
Development and optimization of LFIA The LFIA developed in the present study is based on the competitive format in which polyclonal IgY antibody, showing recognition specificity toward both FB1 and FB2 (Do et al, 2016), is conjugated to gold nanoparticle (see underlying data (Tran et al, 2019))

Summary

Introduction

Y antibody against fumonisin B1 (FB1) and B2 (FB2) was applied for the development of a competitive lateral flow immunoassay detecting these mycotoxins in maize grains with the limit of detection of 4000 μg/kg, which corresponds to the maximum residue limit adopted by the European Commission. To this end, factors affecting the test performance including nitrocellulose membrane type, dilution factor of maize homogenates in running buffer, amount of detection conjugate, and incubation time between detection conjugate and samples were optimized.

Objectives

Methods

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Conclusion