Abstract
Multicomponent analysis has presented a great challenge in pharmaceutical quality control analysis. Thus, in the present work, a selective, accurate and precise liquid chromatographic method (HPLC) has been developed for the simultaneous determination of levodopa and carbidopa in bulk and tablet form. The best resolution was achieved on a C18 column (5 µm particle size, 250 mm×4.6 mm I.D.) using a mixture of phosphate buffer pH 2.8 and acetonitrile (95: 5 v/v) as the mobile phase pumped at a flow rate of 1 ml/min. The constructed calibration curves were linear in the concentration range (25˗250 μg/ml and 2.5 – 25 μg/ml for levodopa and carbidopa, respectively) with correlation coefficients close to 1.0. The accuracy and the precision of the developed method were very good (RSD ˂ 2%). The validity of the proposed method was confirmed through the statistical comparison of the obtained data with those of the official USP method. The developed method proved to be accurate and valid for the simultaneous analysis of levodopa and carbidopa.
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