Abstract
An HPLC-DAD separation method for the simultaneous quantification of ten compounds from Moringa oleifera plant was developed. The method was validated with pure solvent and different matrices of M. oleifera products. This method was found to be linear in the concentration range of 1 to 10 mg L−1 for all the compounds in the solvent and from 3 to 10 mg kg−1 in the different matrices. The correlation coefficients ranged between 0.9900 and 0.9999. Intra-day and inter-day variability showed that the developed method is both repeatable and precise with percent relative standard deviation values less than 10% and 20%, respectively. Limits of detection ranged between 0.06 and 0.8 mg L−1 for the solvent and 0.1–1.5 mg kg−1 for the matrices, while the limit of quantification ranged between 0.2 and 2.8 mg L−1 and 0.4–4.8 mg kg−1, respectively. The validated method was applied successfully to thirty-two different M. oleifera products, whereby all ten compounds were detected in one of the samples. Principal component analysis was used to assess the correlation and variance between the products. Variations were observed in products from different regions and from different manufacturers.
Highlights
Moringa oleifera Lam. is the most cultivated species in the Moringa genus
The recoveries in different matrices had acceptable random errors, as indicated by the percent relative standard deviation (%RSD) values which were below the 20% threshold as stipulated by the EU Commission Decision 2002/657/EC directive [18]. These results indicate that the method is accurate
Figure S2) where these are correlated at a lower level. These results prove the need for quality control methods for the products in the market
Summary
Moringa oleifera Lam. is the most cultivated species in the Moringa genus. It is a valued natural product because of its many uses and it has earned names such as the miracle tree and the magic bullet. All parts of the plant are useful in some way or another, either medical, nutritional, or cosmetic [1]. The plant is used traditionally to treat cholera, diabetes, skin infections, respiratory problems, and ear infection, among others [1,2]. The seeds of the plant are used to purify water, as demonstrated in studies reported by Ndabigengesere et al, Sanchez-Martin et al and Suhartini et al [3,4,5]. Its popularity has led to commercialization of products prepared from the plant
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