Abstract
An enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of gentamicin in human blood serum was developed. Peculiarities of the adsorption on the microtitre plate surface of the gentamicin-protein conjugate were investigated. Different conditions of the competition stage of the analysis were studied and conditions for gentamicin monitoring in human blood serum in the clinical range were optimized. The matrix effect on the assay results, the specificity of the analytical system and the stability of the reagents were examined. The method permits gentamicin concentrations to be determined in human blood serum, diluted 1/1000, in the linear range from 1 to 30 ng/mL. The assay is characterized by high sensitivity (0.5 ng/mL), good reproducibility (CV < 12%) and good correlation with PFIA (r = 0.943).
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
