Abstract
An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for the detection of fenpropathrin was developed. Two haptens, FPa (α-carboxy-3-phenoxyphenyl-2,2,3,3-tetramethyl-cyclopropane-carboxylate) and FPb (α-(N-butyrical)-3-phenoxybenzyl-2,2,3,3-tetramethyl-cyclopropane-carboxylate), were synthesised and conjugated with ovalbumin (OVA) by the mixed anhydride method as coating antigens (FPa–OVA and FPb–OVA), and the hapten FPb was conjugated to bovine serum albumin (BSA) by the carbodimide method to produce an immunogen (FPb–BSA). Polyclonal antibody against fenpropathrin was raised for screening the more sensitive coating antigen. Under optimised assay conditions, the 50% inhibitory concentration (IC50) was 0.34±0.090 mg/L and the limit of detection (LOD) was 0.0093±0.00065 mg/L. The cross-reactivities with other pyrethroids, such as deltamethrin, cypermethrin, fenvalerate and cyhalothrin, were all lower than 0.1%. Water samples spiked with different concentrations of fenpropathrin (0.01–1.0 mg/L) were analysed according to this method. The ic-ELISA developed could successfully be applied to residue analysis of fenpropathrin in aquatic.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
