Abstract
A sensitive immunoassay system was developed for the quantitation of imazaquin, the active ingredient for SCEPTER herbicide. The assay required a polyclonal antibody generated by immunizing rabbits with a bovine serum albumin conjugate of 5-(2-formylvinyl)-2-(4-isopropyl-4-methyl-5-oxo-2-imidazolin-2-yl)benzoic acid. A competitive ELISA system employing horseradish peroxidase conjugated 6-isothiocyanatoimazaquin as a competing molecule was shown to be best for the quantitation of imazaquin
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