Abstract
The development of gold disk electrodes modified with single-stranded PNA (peptide nucleic acid) for the determination of important DNA sequences is described. The performance of anionic and cationic redox indicators for the generation of current signal is compared. It is shown that the use of cationic redox marker, namely hexaaminoruthenium(III) chloride results in a significantly better analytical parameters, as compared to measurements with other tested markers. The optimized sensor distinguishes itself with high selectivity toward complementary DNA sequences, with almost no electrochemical response toward DNA with one base mismatch. Developed PNA-based sensor was successfully used for detection of DNA sequence responsible for sickle cell anemia.
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