Abstract
Based on Cisbio Bioassays' patented HTRF® technology, HTRF Transcreener® ADP assay is a universal method for identifying and characterizing the phosphotransferase activity of ATPases and kinases. ADP is directly detected by a specific monoclonal antibody labeled with europium cryptate and correlates closely with the amount of phosphorylated substrate in a kinase assay. Here we describe a typical four-step assay development with the Eg5 ATPase using the HTRF Transcreener ADP assay.
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