Development of an Effective Chain Elongation Process From Acidified Food Waste and Ethanol Into n-Caproate.

Mark Roghair,David P B T B Strik,Ruud A Weusthuis,Marieke E Bruins,Yuchen Liu,Cees J N Buisman

doi:10.3389/fbioe.2018.00050

Mark Roghair, David P B T B Strik + Show 4 more

Open Access

https://doi.org/10.3389/fbioe.2018.00050

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Abstract

Introduction: Medium chain fatty acids (MCFAs), such as n-caproate, are potential valuable platform chemicals. MCFAs can be produced from low-grade organic residues by anaerobic reactor microbiomes through two subsequent biological processes: hydrolysis combined with acidogenesis and chain elongation. Continuous chain elongation with organic residues becomes effective when the targeted MCFA(s) are produced at high concentrations and rates, while excessive ethanol oxidation and base consumption are limited. The objective of this study was to develop an effective continuous chain elongation process with hydrolyzed and acidified food waste and additional ethanol.Results: We fed acidified food waste (AFW) and ethanol to an anaerobic reactor while operating the reactor at long (4 d) and at short (1 d) hydraulic retention time (HRT). At long HRT, n-caproate was continuously produced (5.5 g/L/d) at an average concentration of 23.4 g/L. The highest n-caproate concentration was 25.7 g/L which is the highest reported n-caproate concentration in a chain elongation process to date. Compared to short HRT (7.1 g/L n-caproate at 5.6 g/L/d), long HRT resulted in 6.2 times less excessive ethanol oxidation. This led to a two times lower ethanol consumption and a two times lower base consumption per produced MCFA at long HRT compared to short HRT.Conclusions: Chain elongation from AFW and ethanol is more effective at long HRT than at short HRT not only because it results in a higher concentration of MCFAs but also because it leads to a more efficient use of ethanol and base. The HRT did not influence the n-caproate production rate. The obtained n-caproate concentration is more than twice as high as the maximum solubility of n-caproic acid in water which is beneficial for its separation from the fermentation broth. This study does not only set the record on the highest n-caproate concentration observed in a chain elongation process to date, it notably demonstrates that such high concentrations can be obtained from AFW under practical circumstances in a continuous process.

Highlights

Medium chain fatty acids (MCFAs), such as n-caproate, are potential valuable platform chemicals
Acidified food waste (AFW) and ethanol were fed to a continuous biological chain elongation process, resulting in production of MCFAs (n-caproate, isocaproate, n-heptanoate and n-caprylate). n-Caproate, the dominant MCFA, was produced (5.5 ± 0.4 g/L/d) at a high steady state concentration of 23.4 ± 1.0 g/L
This was observed at long hydraulic retention time (HRT) (4 d) from day 28 through day 58 (Figure 1)

Summary

Introduction

Medium chain fatty acids (MCFAs), such as n-caproate, are potential valuable platform chemicals. Continuous chain elongation with organic residues becomes effective when the targeted MCFA(s) are produced at high concentrations and rates, while excessive ethanol oxidation and base consumption are limited. Conclusions: Chain elongation from AFW and ethanol is more effective at long HRT than at short HRT because it results in a higher concentration of MCFAs and because it leads to a more efficient use of ethanol and base. The conversion of VFAs into MCFAs with ethanol as electron donor is done by chain elongating micro-organisms (e.g., Clostridium kluyveri) that use the reverse β-oxidation pathway. In this pathway, 1 additional mole of ethanol is oxidized into acetate for every 5 chain elongation reactions (Equation 1) (Seedorf et al, 2008)

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